H2N2V1, Main, Exploration, bibRecord, 000234

Towards the Electrochemical Diagnostic of Influenza Virus: Development of Graphene-Au Hybrid Nanocomposite Modified Influenza Virus Biosensor Based on Neuraminidase Activity

Identifieur interne : 000234 ( Main/Exploration ); précédent : 000233; suivant : 000235

Towards the Electrochemical Diagnostic of Influenza Virus: Development of Graphene-Au Hybrid Nanocomposite Modified Influenza Virus Biosensor Based on Neuraminidase Activity

Auteurs : Ülkü Anik [Turquie] ; Yudum Tepeli [Turquie] ; Maher Sayhi [Tunisie] ; Jihene Nsiri [Tunisie] ; Mohamed Diouani [Tunisie]

Source :

Analyst [ 0003-2654 ] ; 2017-12.

RBID : Hal:pasteur-02020220

Abstract

An effective electrochemical influenza A biosensor based on a graphene-gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum albumin addition and immobilization times of fetuin A and PNA lectin, the analytical characteristics of the influenza A biosensor were investigated. As a result, a linear range between 10-8 U mL-1 and 10-1 U mL-1 was found with a relative standard deviation value of 3.23% (for 10-5 U mL-1 of N, n:3) and a limit of detection value of 10-8 U mL-1 N. The developed biosensor was applied for real influenza virus A (H9N2) detection and very successful results were obtained.

Url:

https://hal-riip.archives-ouvertes.fr/pasteur-02020220

DOI: 10.1039/c7an01537b

Affiliations:

Tunisie, Turquie

Links toward previous steps (curation, corpus...)

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<front><div type="abstract" xml:lang="en">              <p>An effective electrochemical influenza A biosensor based on a graphene-gold (Au) hybrid nanocomposite modified Au-screen printed electrode has been developed. The working principle of the developed biosensor relies on the measurement of neuraminidase (N) activity. After the optimization of experimental parameters like the effect of bovine serum albumin addition and immobilization times of fetuin A and PNA lectin, the analytical characteristics of the influenza A biosensor were investigated. As a result, a linear range between 10-8 U mL-1 and 10-1 U mL-1 was found with a relative standard deviation value of 3.23% (for 10-5 U mL-1 of N, n:3) and a limit of detection value of 10-8 U mL-1 N. The developed biosensor was applied for real influenza virus A (H9N2) detection and very successful results were obtained.</p>
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Towards the Electrochemical Diagnostic of Influenza Virus: Development of Graphene-Au Hybrid Nanocomposite Modified Influenza Virus Biosensor Based on Neuraminidase Activity

Towards the Electrochemical Diagnostic of Influenza Virus: Development of Graphene-Au Hybrid Nanocomposite Modified Influenza Virus Biosensor Based on Neuraminidase Activity

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Abstract

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Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri